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Aldolase A‐HBsAg interaction and its effect on ultraviolet radiation induced apoptosis in 293FT cells
Author(s) -
Pan JinShui,
Zhou Fei,
Xie ChenXi,
Cai JiaYan,
Chen JianMin,
Zhang ZhiPing,
Dong Jing,
Xu HongZhi,
Shi HuaXiu,
Ren JianLin
Publication year - 2010
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2010.06237.x
Subject(s) - hepatitis b virus , hbsag , immunoprecipitation , medicine , apoptosis , aldolase a , antibody , virology , microbiology and biotechnology , cancer research , biochemistry , virus , immunology , biology , enzyme
Background and Aim: Hepatitis B virus (HBV) infection poses great challenges to humans, claiming one million lives annually worldwide. Solid data have related HBV to hepatocellular carcinoma. Methods: In the present research, we verified the interaction between surface protein (HBs) encoded by HBV and aldolase A (ALDA) using yeast two‐hybrid, mammalian two‐hybrid, co‐immunoprecipitation, GST pull‐down and laser scanning confocal. Results: Anti‐ALDA antibody precipitated Gal4‐HBs fusion protein in the presence of HBs. Anti‐HBs antibody precipitated p65ΔN‐ALDA only in the presence of ALDA. Small HBs could be pulled down by GST‐ALDA. Cells transfected with pCMV‐AD‐ALDA showed a protection from ultraviolet radiation‐induced apoptosis (21.3% ± 1.3% for ALDA, 35.4% ± 2.1% for control, P < 0.05). Conclusions: An interaction does exist between ALDA and HBs. The S region within HBs is sufficient for binding ALDA. In addition, ALDA conferred protection to ultraviolet radiation‐induced apoptosis, and this effect was enhanced by the interaction between HBs and ALDA.