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Protecting effects of a large dose of dexamethasone on spleen injury of rats with severe acute pancreatitis
Author(s) -
Xiping Zhang,
Ruiping Zhang,
Binyan Yu,
Li Zhou,
Hanqing Chen,
Wei Zhu,
Rongchao Ying,
Jing Ye,
Wenqin Yuan,
Jinjin Bai
Publication year - 2010
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2009.05999.x
Subject(s) - spleen , dexamethasone , medicine , acute pancreatitis , pathological , pancreatitis , apoptosis , endocrinology , inflammation , survival rate , gastroenterology , biology , biochemistry
Background and Aims: To explore the protecting effects and mechanisms of dexamethasone on spleen injury in rats with severe acute pancreatitis (SAP). Methods: The rats were randomly divided into a model control group, treated group and sham‐operated group. The contents of plasma endotoxin, serum NO, phospholipase A 2 enzyme (PLA 2 ) and endothelin‐1 (ET‐1) were determined. The mortality rate, pathological changes and changes of Bax and Bcl‐2 protein expression levels and apoptotic indexes in the spleen of rats were observed in all groups, respectively, at 3, 6 and 12 h after operation. Results: Although the survival rate was significantly higher in the treated group than in the model control group, there was no significantly different between them ( P > 0.05). The expression levels of Bax and Bcl‐2 proteins and apoptotic indexes were significantly higher in the treated group than in the model control group at different time points ( P < 0.05 or P < 0.01) while other blood indexes contents and pathological severity scores of spleen were significantly lower in the treated group than in the model control group ( P < 0.05, P < 0.01 or P < 0.001). Conclusion: Dexamethasone can protect spleen from injury during SAP mainly by reducing the content of inflammatory mediators in blood.