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IL‐18 serum level and IL‐18 promoter gene polymorphism in Iranian patients with gastrointestinal cancers
Author(s) -
Haghshenas Mohammad Reza,
Hosseini Seyed Vahid,
Mahmoudi Mahmoud,
SaberiFirozi Mehdi,
Farjadian Shirin,
Ghaderi Abbas
Publication year - 2009
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2009.05791.x
Subject(s) - medicine , gastroenterology , colorectal cancer , genotyping , genotype , stomach , stomach cancer , single nucleotide polymorphism , gastrointestinal cancer , haplotype , allele , case control study , allele frequency , cancer , gene , biology , genetics
Background and Aim: Interleukin (IL)‐18 level and association of its two promoter gene polymorphisms at −607C/A and −137G/C positions were investigated in Iranian patients with gastrointestinal (GI) cancers. Methods: 232 cases of GI cancers and 312 healthy controls were enrolled. Serum level of IL‐18 was measured by enzyme linked immunosorbent assay (ELISA) and genotyping of IL‐18 gene polymorphisms were assessed by allele‐specific polymerase chain reaction (PCR). Results: There was a significant difference in the frequency of −137 G/C genotype between patients with stomach or colorectal cancers and control group. In patients with colorectal cancer, the frequency of the −607AA/−137GC genotype combination in unwell‐differentiated cases was more than those with well‐differentiated cancer. Haplotype analysis showed that in patients with stomach cancer −607C/−137C and −607A/−137G and in patients with colorectal cancer −607C/−137C were decreased compared with control group, and this difference reached statistical significance. Serum analysis revealed that the mean IL‐18 serum level in stomach and colorectal cancer before and after surgical operation was significantly higher than healthy volunteers. Postoperative IL‐18 level for all patients with colorectal cancer was significantly decreased compared with the levels before surgery. Conclusion: Results of this investigation suggests that Single Nucleotide Polymorphism (SNP) at position −137 G/C and haplotype frequency may play a role in predisposition of Iranian patients to stomach and colorectal cancers. In addition, increasing serum IL‐18 level may have clinical importance as a diagnostic marker in patients with stomach and colorectal cancer.