Premium
Responses of human sling and clasp fibers to cholecystokinin (CCK) and gastrin through CCK receptors
Author(s) -
Liu JunFeng,
Gao LiPing,
Wen ShiWang,
Lu HuiLai,
Zhang Jian,
Sun Jian,
Zhang ShaoWei,
Wang QiZhang
Publication year - 2008
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2008.05327.x
Subject(s) - cholecystokinin , sling (weapon) , cholecystokinin receptor , medicine , gastrin , receptor , antagonist , contraction (grammar) , agonist , endocrinology , receptor antagonist , surgery , secretion
Background and Aim: Cholecystokinin (CCK) and gastrin exert their influences via CCK receptors. This research was conducted to look at the responses of the sling and clasp fibers forming the human lower esophageal sphincter (LES) to CCK and gastrin, and the role of CCK receptors in the responses. Methods: Muscle strips of sling and clasp fibers from the LES were obtained from patients undergoing subtotal esophagectomy. Isometric tension responses of the strips to CCK‐8 and gastrin‐17 were studied, and the maximum effect (E max ) for each agonist was derived. CCK‐A receptor antagonist, CR1409 and CCK‐B antagonist, CR2945 were applied to sling and clasp fibers and their pK B values were calculated. Results: Sling fibers produced significant contractions following exposure to CCK‐8 and gastrin‐17, while clasp fibers had less responses to the two agents. CR1409 and CR2945 inhibited responses of sling to CCK‐8 in a concentration‐dependent fashion. The inhibition effects of the two antagonists on clasp fibers were not measurable because there was a mild contraction of the fiber in response to CCK‐8. Conclusion: The contractions generated by sling fibers following exposure to CCK and gastrin are greater than that produced by clasp fibers. CCK‐A receptors are more important for the generation of contractions by the sling fibers, whereas both CCK‐A and CCK‐B receptors are involved in the functional regulation of the clasp fibers. [Corrections added after online publication 28 April 2008: in the Background and Aims section of the preceding abstract, all instances of ‘CKK’ were corrected to ‘CCK’. In the final sentence of the abstract ‘CCKA’was corrected to ‘CCK‐A’. In the article title ‘(CKK)’ was corrected to ‘(CCK)’.]