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Gene expression of AGS cells stimulated with released proteins by Helicobacter pylori
Author(s) -
Kim Nayoung,
Park WoongYang,
Kim Jung Mogg,
Park Ji Hyun,
Kim Joo Sung,
Jung Hyun Chae,
Song In Sung
Publication year - 2008
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2007.05241.x
Subject(s) - helicobacter pylori , microbiology and biotechnology , downregulation and upregulation , gene expression , gene , signal transducing adaptor protein , biology , biochemistry , genetics
Background and Aim: Interactions between released proteins by Helicobacter pylori ( H. pylori ) and the cells of gastric epithelium to which it adheres may contribute to gastric inflammation and epithelial damage. The present study was performed to evaluate the gene expression of AGS gastric cancer cells stimulated with released proteins by H. pylori . Methods: Gene expression of AGS cells to the stimulation by H. pylori ‐released proteins (G27 strain) were monitored using oligonucleotide microarrays. Results: Eighty‐eight genes (0.88%) and eight genes (0.08%) were up‐ or downregulated, respectively, by treating AGS cells with H. pylori ‐released proteins but not by H. pylori adhesion after 12 h of coculture. Out of the selected 40 up‐ and five downregulated genes, 29 upregulated genes classified as general RNA polymerase II transcription factor activity ( GTF2B , PPARGC1A ), SH3 / SH2 adaptor activity ( CRKL ), transferase activity ( ACLY , CRKL , PIGC , PLK4 ), and oxidoreductase activity ( IDH1 ) were confirmed to be upregulated by released proteins and not by H. pylori adhesion by real‐time reverse transcription–polymerase chain reaction. When the concentrated H. pylori ‐cultured supernatant prepared by our protocol was treated by boiling, the upregulations of 26 of these 29 genes (89.7%) except for CD160 , ZNF268 , and PSAT1 disappeared. This confirmed that most of these upregulations were caused by released proteins. Conclusion: Host genes involving transcription, signaling and stress are significantly modulated by the proteins released by H. pylori . This might strengthen the gastroduodenal pathogenesis induced by H. pylori .