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Cholecystokinin receptor A gene polymorphism in gallstone disease and gallbladder cancer
Author(s) -
Srivastava Anvesha,
Pandey Sachchida Nand,
Dixit Manjusha,
Choudhuri Gourdas,
Mittal Balraj
Publication year - 2008
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2007.05170.x
Subject(s) - gastroenterology , medicine , gallbladder , gallbladder cancer , gallstones , genotype , cholecystokinin , odds ratio , gallbladder disease , gnas complex locus , gene , receptor , biology , genetics
Background and Aim: Gallbladder carcinoma (GBC) usually arises in the background of gallstone disease which may be causatively related to decreased gallbladder contractility. Cholecystokinin receptor A ( CCK‐AR ) mediates signals resulting in gallbladder contraction. Deteriorating gallbladder contraction promotes gallstone formation. A common genetic polymorphism of CCK‐AR may be causatively associated with the risk of gallstone and GBC. This study aimed to understand the association of CCK‐AR Pst I polymorphism in gallstone disease with gallbladder cancer. Method: This study included 165 gallstone patients, 139 GBC patients, and 190 healthy subjects. Genotyping was done using the polymerase chain reaction–restriction fragment length polymorphism (PCR‐RFLP) method. Results: The frequency of the A1A1 genotype of CCK‐AR was significantly higher in gallstone patients than healthy individuals ( P = 0.008 odds ratio [OR] = 2.25, and 95% confidence interval [CI]:1.2–4.1). However, there was a significant difference in the frequency of A1A1 genotype when gallstone patients were compared to GBC patients ( P = 0.041, OR = 0.49, and 95% CI: 0.3–0.9). On stratification of GBC patients according to presence or absence of gallstones, GBC patients without stones were compared to controls and GBC patients with stones were compared to stone patients; however, no significant differences in frequencies were observed. Conclusion: The results suggest that the A1A1 genotype of CCK‐AR is an independent genetic risk factor for gallstone disease and does not modulate the susceptibility of gallbladder cancer.