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Experimental investigation of the role of endothelin‐1 in idiopathic portal hypertension
Author(s) -
Yamaguchi Emi,
Yamanoi Akira,
Ono Takashi,
Nagasue Naofumi
Publication year - 2007
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2006.04822.x
Subject(s) - medicine , portal hypertension , endothelin receptor , gastroenterology , receptor , cirrhosis
Background and Aim: The authors' previous report revealed that endothelin‐1 might be released from B lymphocytes in cirrhotic patients with hypersplenism. Other investigators have shown that persistent exposure to environmental contaminants including arsenic might induce idiopathic portal hypertension. The aim of this study was to experimentally identify how endothelin‐1 is involved in the development of idiopathic portal hypertension and which cells produce endothelin‐1 in the spleen. Methods: Portal pressure and venous endothelin‐1 concentrations were measured in rats that were given sodium arsenate orally for long periods, and endothelin‐1 expression levels in the spleen were assessed by staining. In a second experiment, B and T lymphocytes and monocyte‐derived macrophages cultured from healthy human peripheral blood were stimulated with sodium arsenite, sodium arsenate, lipopolysaccharide and interferon‐γ. Endothelin‐1 concentrations in the supernatants were measured by ELISA. Results: Arsenic exposure gradually increased portal pressure and venous endothelin‐1 levels in rats. Endothelin‐1 concentration in the supernatant did not change in every cell type stimulated with arsenic, but it increased in B lymphocytes and monocyte‐derived macrophages treated with lipopolysaccharide and interferon‐γ. Conclusions: The in vivo study indicated that arsenic might elevate portal pressure through mechanisms involving endothelin‐1. In the in vitro study, lipopolysaccharide and interferon‐γ clearly induced endothelin‐1 synthesis not only in monocyte‐derived macrophages but also in B lymphocytes, although arsenic treatment did not affect those cells. This study partially supports the hypothesis that idiopathic portal hypertension might be promoted by endothelin‐1 overproduction from splenic B lymphocytes in response to certain substances. © 2007 The Authors; Journal compilation © 2007 Journal of Gastroenterology and Hepatology Foundation and Blackwell Publishing Asia Pty Ltd