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Gastrointestinal stromal tumors: Usefulness of immunohistochemistry, flow cytometry and fluorescence in situ hybridization
Author(s) -
Fontana Maria Grazia,
Rossi Elisa,
Bassotti Gabrio,
Aquilano Maria Costanza,
Cadei Morris,
Grigolato Piergiovanni,
Villanacci Vincenzo
Publication year - 2007
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2006.04530.x
Subject(s) - cd117 , fluorescence in situ hybridization , gist , immunohistochemistry , pathology , medicine , cd34 , malignancy , aneuploidy , flow cytometry , stromal cell , in situ hybridization , gastrointestinal tract , biology , chromosome , immunology , biochemistry , gene expression , genetics , stem cell , gene
Background and Aims: Gastrointestinal stromal tumors (GIST) constitute a group of primary mesenchymal tumors of the gastrointestinal tract, known for their diversity in clinical behavior and the difficulties in determining malignancy and prognosis. This retrospective study evaluated a series of GIST by means of immunohistochemical techniques, flow cytometry and fluorescence in situ hybridization (FISH). Methods: Nine patients with GIST were analyzed for tumor size, mitotic count and CD117, CD34, MIB‐1 with immunohistochemistry. In addition, the GIST were tested with FISH for chromosomes 8 and 17 and DNA index was evaluated by flow cytometry. Results: The findings confirmed the usefulness of CD117 and CD34 in diagnosing GIST and the prognostic role of MIB‐1, but do not support a correlation between aneuploidy in flow cytometry and poor outcome. The FISH results suggest close follow‐up for patients with benign GIST with a numerical alteration of chromosome 8. The technique could select patients with tumors at high‐risk with aneusomy of chromosome 17. Conclusion: This study shows the possible application of FISH to the evaluation of patients with GIST, in addition to analysis of morphological features.