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Analysis of differential gene expression between chronic hepatitis B patients and asymptomatic hepatitis B carriers
Author(s) -
Tang Cuilan,
Chen Zhi,
Peng Guoping,
Yang Zhenggang,
Zhou Linfu
Publication year - 2007
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2006.04371.x
Subject(s) - suppression subtractive hybridization , asymptomatic carrier , hepatitis b virus , peripheral blood mononuclear cell , virology , gene , dot blot , hepatitis b , biology , in situ hybridization , gene expression , medicine , immunology , virus , asymptomatic , genetics , pathology , cdna library , in vitro
Background and Aims: Chronic hepatitis B virus (HBV) infection remains a serious global health problem, inducing a spectrum of diseases, including asymptomatic HBV carriage (ASC) and chronic hepatitis B (CHB). ASC and CHB represent different immunological states and their prognoses are diverse. To clarify molecular mechanisms underlying the two infection states, the differentially expressed genes between the two states were screened and identified. Methods: Subtracted complementary DNA libraries by suppression subtractive hybridization, dot blot hybridization and quantitative real‐time PCR were used to identify the differentially expressed genes between subjects with CHB and those with ASC. Results: RNA from peripheral blood mononuclear cells from CHB and ASC subjects was subjected to suppression subtractive hybridization and resulted in isolation of subtracted complementary DNA clones. Eighty‐eight randomly sampled clones were rescreened by dot blot hybridization, from which 29 clones were identified as differentially expressed genes. The differential expression of three genes was confirmed by real‐time PCR in 23 subjects with CHB and 21 with ASC. Conclusions: Differentially expressed genes in peripheral blood mononuclear cells between CHB and ASC have been isolated by suppression subtractive hybridization, including some new genes. Of the up‐regulated genes in CHB, most are known to be responsive to inflammatory conditions. These genes might provide clues in elucidating the mechanisms of the two different HBV infection states and designing therapeutic targets for HBV infection.