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Protective role of supplement with foreign Bifidobacterium and Lactobacillus in experimental hepatic ischemia‐reperfusion injury
Author(s) -
Xing HuiChun,
Li LanJuan,
Xu KaiJin,
Shen Tian,
Chen YunBo,
Sheng JiFang,
Chen Yu,
Fu SuZhen,
Chen ChunLei,
Wang JianGuo,
Yan Dong,
Dai FangWei,
Zheng ShuSen
Publication year - 2006
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2006.04306.x
Subject(s) - lactobacillus fermentum , bifidobacterium , lactobacillus , medicine , malondialdehyde , superoxide dismutase , microbiology and biotechnology , immunology , oxidative stress , biology , lactic acid , bacteria , lactobacillus plantarum , genetics
Background and Aim: Intestinal microflora play a crucial role in some severe liver diseases. The purpose of this study was to evaluate the effects of a Lactobacillus strain and a Bifidobacterium strain on ischemia‐reperfusion (I/R) liver injury. Methods: Rats were divided into six groups. Each group received either Bifidobacterium Catenulatum ZYB0401; Lactobacillus Fermentum ZYL0401; a mixture of these two bacterial strains; gentamicin; or saline by daily gavage for 7 days. On the sixth day, all rats, except those in the control group, were subjected to 20 min of liver ischemia. After 22 h of hepatic reperfusion, liver enzymes and histology, malondialdehyde (MDA), superoxide dismutase (SOD), endotoxemia, serum tumor necrosis factor‐α (TNF‐α), intestinal bacteria, intestinal mucosal ultrastructure, and bacterial translocation were studied. Results: All administered bacteria increased intestinal Bifidobacterium and Lactobacillus , decreased endotoxemia ( P < 0.01), alanine aminotransferase (ALT) ( P < 0.01), and markedly ameliorated liver histology and intestinal mucosal ultrastructure. Only rats treated with Bifidobacterium Catenulatum ZYB0401 and Lactobacillus Fermentum ZYL0401 showed reduced incidence of bacterial translocation to the kidney ( P < 0.05), associated with decreased serum TNF‐α and liver MDA ( P < 0.05) and increased liver SOD ( P < 0.05) compared to the I/R group. Gentamicin decreased almost all kinds of intestinal bacteria ( P < 0.01) and decreased ALT ( P < 0.01) and serum TNF‐α, but failed to reduce both endotoxemia and the incidence of bacterial translocation and had no effects on liver MDA and SOD. Conclusion: Bifidobacterium Catenulatum ZYB0401 in combination with Lactobacillus Fermentum ZYL0401 could be useful in restoring intestinal microflora and in preventing liver injury in hepatic I/R of rats.