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Influence of urease activity in the intestinal tract on the results of 13 C‐urea breath test
Author(s) -
Urita Yoshihisa,
Hike Kazuo,
Torii Naotaka,
Kikuchi Yoshinori,
Kanda Eiko,
Kurakata Hidenori,
Sasajima Masahiko,
Miki Kazumasa
Publication year - 2006
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2006.04278.x
Subject(s) - urease , duodenum , medicine , rapid urease test , urea , gastroenterology , urea breath test , ingestion , breath test , stomach , helicobacter pylori , excretion , gastritis , chemistry , biochemistry , helicobacter pylori infection
Background and Aim: A late rise in 13 CO2 excretion in the 13 C‐urea breath test (UBT) should be found when the substrate passes rapidly through the stomach and makes contact with the colonic bacteria. The aim of this study was to evaluate the influence of intestinal urease activity on the results of the UBT. Method: A total of 143 subjects who were diagnosed as Helicobacter pylori negative by serology, histology and rapid urease test were recruited. At the end of endoscopy, the tip of the endoscope was placed to the second part of the duodenum and 20 mL of water containing 100 mg of 13 C‐urea was sprayed into the duodenum. Breath samples were taken at baseline and at 5, 10, 20, 30 and 60 min after administration. Results: Of 143 subjects, breath Δ 13 CO2 values higher than 2.5‰ were detected in six (4.2%), four (2.8%) and five (3.5%) subjects at 20, 30 and 60 min, respectively. There was no subject with high Δ 13 CO2 values at 5 and 10 min. Only one subject had an immediate rise at 60 min. Conclusion: Variability derived from urease activity in the intestinal tract appears to be minimal up to 60 min after ingestion of the test urea.