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Apoptosis in rat jejunal mucosa is regulated partly through the central nervous system, which controls feeding behavior
Author(s) -
LIN TAISAN,
SAKATA HIROYUKI,
OOTANI AKIFUMI,
FUJISE TAKEHIRO,
TSUNADA SEIJI,
AMEMORI SADAHIRO,
DANJO AKIKO,
YOKOYAMA FUMIE,
SAKATA YASUHISA,
IWAKIRI RYUICHI,
TODA SHUJI,
FUJIMOTO KAZUMA
Publication year - 2005
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2005.03921.x
Subject(s) - apoptosis , tunel assay , jejunum , endocrinology , medicine , vagotomy , central nervous system , glucosamine , enteric nervous system , intestinal mucosa , leptin , biology , immunohistochemistry , biochemistry , obesity
Aim: The aim of this study was to investigate whether central nervous system‐related feeding behavior regulates mucosal apoptosis in rat small intestines. Methods: The test solutions used in this study were an H 1 receptor antagonist (chlorpheniramine maleate), 2‐deoxy‐D‐glucose, leptin, and 1‐deoxy‐D‐glucosamine (2‐amino‐1,5‐anhydro‐2‐deoxy‐D‐glucitol). Test solutions were injected into the third cerebroventricles of rats. Feeding behavior and jejunal apoptosis were evaluated both with and without truncal vagotomy. Intestinal apoptosis was evaluated by percentage fragmented DNA, electrophoresis, and TUNEL staining. Results: Chlorpheniramine and 2‐deoxy‐D‐glucose elicited feeding, whereas leptin and 1‐deoxy‐D‐glucosamine suppressed feeding. The test solutions, which elicited feeding (0.24 and 24 µmol/rat of chlorpheniramine and 2‐deoxy‐D‐glucose, respectively), suppressed mucosal apoptosis in the rat jejunum 1 h after cerebroventricular infusion. In contrast, the test solutions, which suppressed feeding (8 and 24 µmol/rat of leptin and 1‐deoxy‐D‐glucosamine, respectively), induced jejunal mucosal apoptosis 3 h after infusion. The effects of the test solutions on feeding behavior and changes in apoptosis were not affected by truncal vagotomy. Conclusion: The central nervous system, which regulates feeding behavior, might control intestinal function through the regulation of intestinal apoptosis.