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Feasibility of a breath test with a substrate of natural 13 C‐abundance and isotope‐selective non‐dispersive infrared spectrometry: A preliminary study
Author(s) -
JONDERKO KRZYSZTOF,
KASICKAJONDERKO ANNA,
SYRKIEWICZTREPIAK DANUTA,
BŁOŃSKAFAJFROWSKA BARBARA
Publication year - 2005
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2005.03920.x
Subject(s) - breath test , ingestion , medicine , isotope ratio mass spectrometry , zoology , postprandial , mass spectrometry , nuclear medicine , surgery , chromatography , chemistry , biology , helicobacter pylori , insulin
Background and Aims: Isotope‐selective non‐dispersive infrared spectrometry (NDIRS) is a cheaper alternative to isotope ratio mass spectrometry (IRMS). We checked on the efficiency of NDIRS to perform a breath test with naturally 13 C‐enriched maize starch. Methods: Fifty‐six healthy volunteers were examined. After a basal fasted sample of their expiratory air was collected, the subjects ate a test breakfast of 50 g (CF50 group, n = 9) or 100 g (CF100 group, n = 47) cornflakes with 175 g yoghurt. For 6 h thereafter, exhaled air was collected every 30 min for the NDIRS measurement of 13 CO 2 concentration. Results: The time‐course of the curves of 13 CO 2 concentration resembled those obtained previously with IRMS. A dose‐response to the amount of ingested cornflakes was observed – the maximum postprandial net increment in 13 CO 2 was statistically significantly higher in the CF100 than CF50 group: 4.78 ± 0.13 versus 3.12 ± 0.17‰ ( P < 0.001), whereas the time needed to reach the maximum did not differ after the intake of 50 g (233 ± 13 min) or 100 g (248 ± 9 min) cornflakes. The 5‐h area‐under‐the‐curve of cumulative 13 CO 2 net recovery (AUC 60‐360 ) was statistically significantly greater after ingestion of 100 g compared to 50 g cornflakes (97.6 ± 2.2 versus 61.7 ± 2.2 µmol, P < 0.001) and it also exhibited a stable between‐subject variability. Conclusion: The obtained proof of technical feasibility of a breath test with the use of naturally 13 C‐enriched starch and NDIRS provides background for future research on the clinical usefulness of this method for a non‐invasive assessment of the pancreatic exocrine function.