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Effects of growth factors on the growth and differentiation of mouse fetal liver epithelial cells in primary cultures
Author(s) -
KAWASAKI TAKAKO,
TAMURA SHINJI,
KISO SHINICHI,
DOI YOSHINORI,
YOSHIDA YUICHI,
KAMADA YOSHIHIRO,
SAEKI AYUKO,
SAJI YUKIKO,
MATSUZAWA YUJI
Publication year - 2005
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2005.03812.x
Subject(s) - epidermal growth factor , hepatocyte growth factor , dna synthesis , basic fibroblast growth factor , transforming growth factor , growth factor , hepatocyte , fibroblast growth factor , endocrinology , medicine , biology , heparin binding egf like growth factor , cell culture , microbiology and biotechnology , dna , biochemistry , in vitro , receptor , genetics
Background and Aims: Growth factors (GF) are thought to affect the growth and differentiation of hepatocytes during liver development. However, in the midfetal liver, little is known concerning the role of GF. Methods: The DNA synthesis of fetal liver epithelial cells (FLEC) in monolayer culture and the liver‐specific gene expressions of FLEC in 3‐D culture were examined in medium supplemented with various GF. Results: DNA synthesis of FLEC was higher than that of adult hepatocytes without GF, and was increased by hepatocyte growth factor (HGF), heparin‐binding epidermal growth factor‐like growth factor (HB‐EGF), basic fibroblast growth factor (bFGF), epidermal growth factor (EGF) or transforming growth factor‐alpha (TGF‐α). However, FLEC responded less to GF in terms of DNA synthesis than adult hepatocytes. The liver‐specific gene expressions were increased in the presence of HGF, HB‐EGF, bFGF and EGF. In embryonic day (E) 13.5 FLEC, this increase was more apparent in the presence of HB‐EGF, whereas in E14.5 FLEC, it was more apparent in the presence of HGF. Conclusions: Hepatocyte growth factor, HB‐EGF, bFGF, EGF and TGF‐α increased DNA synthesis of FLEC. HGF, HB‐EGF, bFGF and EGF led to an increase in liver‐specific gene expressions; and their effects on differentiation differ as a function of gestation age.