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Distribution of type V secretory phospholipase A 2 expression in human hepatocytes damaged by liver disease
Author(s) -
ITO MASAMICHI,
ISHIKAWA YUKIO,
KIGUCHI HIDEKO,
KOMIYAMA KAZUO,
MURAKAMI MAKOTO,
KUDO ICHIRO,
AKASAKA YOSHIKIYO,
ISHII TOSHIHARU
Publication year - 2004
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2004.03435.x
Subject(s) - cirrhosis , hepatocyte , medicine , hepatitis , viral hepatitis , liver disease , immunostaining , pathology , immunohistochemistry , biology , immunology , biochemistry , in vitro
Background and Aim: Type V secretory phospholipase A 2 (sPLA 2 ‐V) is a key enzyme in the arachidonate cascade. However, the distribution of sPLA 2 ‐V in human liver has not yet been investigated. In this study, the significance of sPLA 2 ‐V expression in human hepatocytes damaged by liver disease was investigated. Methods: Samples of liver tissue from patients with chronic hepatitis B and C, hepatitis virus‐related liver cirrhosis, and congestive hepatocyte injury were immunostained with antibodies against sPLA 2 ‐V, cyclooxygenase (COX)‐2, hepatitis viral antigens, transforming growth factor (TGF)‐β 1 , interleukin (IL)‐1β and tumor necrosis factor (TNF)‐α. Results: In chronic hepatitis patients, sPLA 2 ‐V‐positive hepatocytes were scattered in the liver lobules, while cyclooxygenase‐2, IL‐1β, and TNF‐α were diffusely expressed. Hepatocytes around necroinflammatory lesions were strongly positive for sPLA 2 ‐V. Some sPLA 2 ‐V‐positive hepatocytes were also positive for viral antigens. TGF‐β 1 was expressed only in fibrotic lesions. The pattern of distribution of these proteins in liver cirrhosis patients was similar to that in chronic hepatitis patients, but sPLA 2 ‐V expression tended to be more intense than in chronic hepatitis. In the congestive liver, sPLA 2 ‐V, COX‐2, and the two cytokines were diffusely expressed in surviving hepatocytes. Conclusions: sPLA 2 ‐V expression in hepatocytes is induced by viral infection, fibrosis, and circulatory disturbance. Immunostaining using sPLA 2 ‐V antibody is useful for the detection of injured hepatocytes in patients with liver diseases.