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Experimental bile‐duct ligation resulted in accumulation of oxidized low‐density lipoproteins in BALB/c mice liver
Author(s) -
CÖMERT MUSTAFA,
TEKİN İSHAK ÖZEL,
AÇIKGÖZ ŞEREFDEN,
ÜSTÜNDAǦ YÜCEL,
UÇAN BÜLENT H,
ACUN ZEKI,
BARUT FIGEN,
SÜMBÜLOǦLU VILDAN
Publication year - 2004
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2004.03400.x
Subject(s) - malondialdehyde , lipid peroxidation , oxidative stress , glutathione , medicine , jaundice , endocrinology , bile duct , cholestasis , chemistry , biochemistry , enzyme
Abstract Background and Aim: Oxidized low‐density lipoproteins (LDL), which are produced during oxidative stress by the process of lipid peroxidation, have also been proposed to have complex roles in many other immuno‐inflammatory mechanisms. It has been shown that bile‐duct ligation results in oxidative stress in the liver of animals. The aim of this study was to investigate if oxidized LDL are produced in the liver tissues of bile‐duct‐ligated mice. Methods: Obstructive jaundice was induced in BALB/c mice by the ligation and division of the common bile duct. Liver concentrations of glutathione and malondialdehyde were measured in the sham‐operated ( n = 10) and bile‐duct‐ligated ( n = 10) mice on the 10th day of obstructive jaundice. The presence of oxidized LDL in the liver tissue sections was evaluated using a special, novel immunofluorescent staining method. The final step was to explore the existence of oxidized LDL under fluorescent microscopy. Results: Compared with sham‐operated mice, jaundiced mice showed significantly higher levels of malondialdehyde and lower concentrations of reduced glutathione in the liver. While there was no staining in the sham‐operated group, bile‐duct ligation resulted in positive oxidized LDL staining in the liver tissues of mice. The present study testifies that bile‐duct ligation results in oxidative stress and enhanced lipid peroxidation in the hepatic tissues of BALB/c mice and moreover, that oxidized LDL accumulate in the liver of mice with experimental obstructive jaundice. Conclusion: Oxidized LDL may be an important and direct indicator of ongoing oxidative stress and enhanced lipid peroxidation in obstructive jaundice. The potential roles of this finding were also discussed, briefly. © 2004 Blackwell Publishing Asia Pty Ltd