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Chromosomal and microsatellite instability in sporadic gastric cancer
Author(s) -
HIYAMA TORU,
TANAKA SHINJI,
YOSHIHARA MASAHARU,
SASAO SHOGO,
KOSE KAZUHIRO,
SHIMA HIDEYUKI,
TUNCEL HANDAN,
UENO YOSHITAKA,
ITO MASANORI,
KITADAI YASUHIKO,
YASUI WATARU,
HARUMA KEN,
CHAYAMA KAZUAKI
Publication year - 2004
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.2004.03369.x
Subject(s) - microsatellite instability , loss of heterozygosity , chromosome instability , cancer , genome instability , cancer research , chromosome , comparative genomic hybridization , biology , microsatellite , gene , genetics , allele , dna , dna damage
Background: Gastric cancer can progress through two pathways of genomic instability: chromosomal (CIN) and microsatellite instability (MSI). It is hypothesized that these two pathways are not always independent and that some tumors show overlap between these two mechanisms. Methods: A total of 98 sporadic gastric cancers were classified based on their MSI status, using microsatellite assay with BAT26. Evidence for CIN was investigated by identifying loss of heterozygosity (LOH) events on chromosome arms, 5q, 10p, 17p, 17q, and 18q, which are regions harboring tumor suppressor genes that are significant in gastric cancer development. Results: Twelve tumors (12%) showed high‐frequency MSI (MSI‐H). Overall, 43 of the tumors (44%) had at least one LOH event, with most frequent chromosomal losses observed on 10p and 18q (30%, respectively), followed by 5q (21%), 17p (14%), and 17q (12%). Interestingly, overlap was observed between CIN and MSI pathways. Of 43 cancers with LOH events, four (9%) were also MSI‐H. It was also found that 48% of cancers without MSI‐H had no LOH events identified, comprising a subgroup of tumors that were not representative of either of these two pathways of genomic instability. Conclusion: These results suggest that molecular mechanisms of genomic instability are not necessarily independent and may not be fully defined by either the MSI or CIN pathways in sporadic gastric cancers.