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Expression of cellular adhesion regulatory molecule in hepatocellular carcinoma
Author(s) -
SUNAGA MASAHIKO,
YOSHIKAWA MASAHARU,
EBARA MASAAKI,
KONDO FUKUO,
KASHIWADA MASAKI,
TAKEMORI TOSHITADA,
SAISHO HIROMITSU
Publication year - 1998
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1998.tb00755.x
Subject(s) - hepatocellular carcinoma , medicine , cell adhesion molecule , cancer research , adhesion , carcinoma , pathology , immunology , chemistry , organic chemistry
Cellular adhesion regulatory molecule (CMAR) enhances the adhesiveness of cells to collagen and laminin and is considered to be a candidate anti‐oncogene. The purpose of the present study was to investigate the relationship between the expression of CMAR and clinical features of hepatocellular carcinoma (HCC). Small amounts of liver tissue were obtained from HCC and non‐cancerous portions of the liver in 29 patients and from normal liver in seven patients with metastatic liver tumour by biopsy under ultrasound guidance. RNA was extracted with acid guanidinium thiocyanate‐phenol‐chloroform. Expression of CMAR was assessed by quantitative PCR using β‐actin as an internal standard. A 4 b.p. insertion polymorphism at nucleotide 241 of the CMAR coding region was then investigated using extracted RNA to assess the relationship between the expression of variant mRNA of CMAR and HCC carcinogenesis. The relative expression of CMAR was significantly reduced in HCC compared with non‐cancerous and normal livers and had a relationship with certain clinical background factors. The reduced expression of CMAR was thought to be closely associated with the progression of HCC. However, the 4 b.p. insertion polymorphism pattern of CMAR was the same between HCC and non‐cancerous liver in all cases in which it was found. These results suggest that progression of HCC may be predicted based on the relative expression of CMAR.