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Induction of CINC (interleukin‐8) production in rat liver by non‐parenchymal cells
Author(s) -
OHKUBO KEIJI,
MASUMOTO TOSHIKAZU,
HORIIKE NORIO,
ONJI MORIKAZU
Publication year - 1998
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1998.tb00716.x
Subject(s) - lipopolysaccharide , parenchyma , cytokine , medicine , tumor necrosis factor alpha , hepatocyte , sinusoid , in vitro , kupffer cell , interleukin , endocrinology , pathology , biology , biochemistry
The production of interleukin‐8 (CINC: cytokine‐induced neutrophil chemo‐attractant) from different cell populations in the rat liver was studied and cells related to the initiation of CINC production in lipopolysaccharide (LPS)‐injected endotoxaemic rats were characterized. Sinusoidal endothelial cells (16.4 ± 10.6 ng/mL) produced significantly higher amounts of CINC in 24 h primary cultures compared with hepatocytes (0.9 ± 0.9 ng/mL; P < 0.05) and Kupffer cells (6.5 ± 5.1 ng/mL; P < 0.05). Lipopolysaccharide, tumour necrosis factor‐α (TNF‐α), and interleukin‐1α (IL‐1α) stimulated different liver cell populations to produce CINC; LPS mainly stimulated Kupffer cells, TNF‐α stimulated hepatocytes and IL‐1α stimulated all three types of cells. Intraperitoneal injection of LPS (4 mg/kg) caused CINC accumulation in non‐parenchymal cells of the rat liver within 1 h of injection, as shown by immunohistochemical staining. In contrast, CINC‐positive hepatocytes were not seen until 3 h after injection of LPS. Ethanol was not a direct inducer of CINC production by rat hepatocytes in vitro. These findings strongly suggest that non‐parenchymal liver cells, including sinusoidal endothelial cells, are the main source of CINC. Our data also suggest that during endotoxaemia, CINC production is initiated by non‐parenchymal cells and this is followed by production from hepatocytes.