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A protease inhibitor attenuates gastric erosions and microcirculatory disturbance in the early period after thermal injury in rats
Author(s) -
YOSHIDA MASASHI,
WAKABAYASHI GO,
ISHIKAWA HIDEKI,
OTANI YOSHIHIDE,
SHIMAZU MOTOHIDE,
KUBOTA TETSURO,
KUMAI KOICHIRO,
KUROSE IWAO,
MIURA SOICHIRO,
ISHII HIROMASA,
KITAJIMA MASAKI
Publication year - 1998
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1998.tb00553.x
Subject(s) - medicine , microcirculation , proteases , serine protease , distilled water , pharmacology , in vivo , protease , anesthesia , chemistry , biochemistry , biology , enzyme , chromatography , microbiology and biotechnology
The effects of camostat mesilate, a synthetic serine protease inhibitor on gastric microcirculation and active oxygen species generated by leucocytes from the gastric and jugular veins in the early period after thermal injury were assessed. Male Wistar rats were anaesthetized and a 30% full skin‐thickness dorsal burn was inflicted. Camostat mesilate (100 mg/kg) was dissolved in distilled water and administered orally to rats 40 min before thermal injury (the camostat group). The control animals (the vehicle group) were administered distilled water orally. Rolling leucocytes as well as Monastral blue B deposits in venules were observed using in vivo microscopy. Active oxygen species were measured by chemiluminescence. Camostat mesilate decreased the total length of gastric erosion, venular deposits of Monastral blue B, and rolling of leucocytes in venules, and relatively increased luminol‐dependent chemiluminescence activity generated by zymosan‐stimulated leucocytes 15 min after thermal injury. These results suggest that serine proteases are involved in the formation of gastric erosions and gastric microcirculatory disturbance in the early period after thermal injury.