Premium
Cytotoxic T lymphocyte clone specific for autologous human hepatocellular carcinoma cell line SUHC‐1
Author(s) -
YOSHIZAWA KANAME,
OTA MASAO,
KIYOSAWA KENDO
Publication year - 1998
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1998.tb00541.x
Subject(s) - medicine , clone (java method) , cytotoxic t cell , hepatocellular carcinoma , lymphocyte , cell culture , cancer research , carcinoma , t lymphocyte , immunology , oncology , pathology , immune system , dna , in vitro , biology , genetics
We established a cytotoxic T lymphocyte (CTL) clone directed against an autologous hepatocellular carcinoma (HCC) cell line SUHC‐1 which had been established in our department from a patient with HCC associated with hepatitis C virus infection. The CTL clone lysed autologous SUHC‐1 cells but did not lyse autologous Epstein‐Barr (EB) virus‐transformed B cells, natural killer (NK) cell‐sensitive erythroleukaemia cell line K562, the NK‐resistant B cell line Daudi, or allogeneic HCC cell lines, Hep‐G2, Hep‐3B, Mahlavu and PLC/PRF/5. The CTL clone expressed CD3 and CD8 molecules. The cytotoxic activity of the clone was inhibited by anti‐CD3, anti‐CD8 and anti‐histocompatibility antigen (HLA) class I monoclonal antibodies. These results indicated that the CTL clone recognized HCC tumour antigen in an HLA class I‐restricted manner. Furthermore, we investigated the T cell receptor (TCR) gene usage of the CTL clone. The CTL clone expressed TCRαβ. We searched for expression of TCR variable (V) α and β regions and sequenced complementary determining region (CDR) 3 of the clone. The clone expressed Vα14, junctional (J) region α9.7 and Vβ7, Jβ2.1. The amino acid sequence of the N region of the α chain was S‐P‐G‐G‐G‐G‐A‐D‐G‐L‐T and of the N‐D‐N region of the β chain was S‐W‐T‐G‐A‐S‐T‐D‐T‐Q‐Y. These results suggested that HLA class I‐restricted CTL play an important role in the elimination of human HCC cells.