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Virulence‐associated genes as markers of strain diversity in Helicobacter pylori infection
Author(s) -
ITO AKIRA,
FUJIOKA TOSHIO,
KODAMA KAORU,
NISHIZONO AKIRA,
NASU MASARU
Publication year - 1997
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1997.tb00532.x
Subject(s) - caga , helicobacter pylori , virulence , gastritis , gastroenterology , microbiology and biotechnology , medicine , helicobacter , spirillaceae , polymerase chain reaction , gene , biology , genetics
To establish a marker of strain diversity of Helicobacter pylori , a genetic examination was performed based on the detection rates by PCR of cagA and vacA , which are known to be virulence‐associated genes. The test strains were obtained from 70 patients suffering from gastric ulcer (GU), 82 patients with duodenal ulcer (DU) and 48 patients with gastritis (GS). Fragments located in the three different regions of vacA were amplified; V1 being the upstream portion, V2 the mid‐portion and V3 the downstream portion. For cagA , the detection rates were 70% for GU, 79% for DU and 50% for GS, showing a significantly higher rate for DU than for GS ( P = 0.0005). With V1, the detection rates were 90% for GU, 90% for DU and 69% for GS, giving a significantly higher rate for GU than for GS ( P = 0.0036) and also giving a significantly higher rate for DU than for GS ( P = 0.0019). With V2, the detection rates were 60% for GU, 70% for DU and 44% for GS, giving a significantly higher rate for DU than for GS ( P = 0.0024). The differences in vacA gene polymorphism were closely related to the evidence of gastroduodenal ulcers in H. pylori infection. Furthermore, the detection rates of cagA and polymorphisms of vacA by PCR could be used as markers of strain diversity in H. pylori ‐induced gastroduodenal ulcer.