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Analysis of interleukin‐8 secretion induced by Helicobacter pylori from the gastric epithelial cell line MKN45: A mechanism independent of the intensity of cytotoxicity
Author(s) -
TAKAGI ATSUSHI,
KAMIYA SHIGERU,
KOGA YASUHIRO,
OHTA UKI,
KOBAYASHI HIROHISA,
SHIRAI TAKAYUKI,
HARASAWA SHIGERU,
MIWA TAKESHI
Publication year - 1997
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1997.tb00444.x
Subject(s) - secretion , cytotoxicity , helicobacter pylori , cytotoxic t cell , interleukin 8 , cell culture , medicine , microbiology and biotechnology , biology , immunology , cytokine , in vitro , biochemistry , genetics
Interleukin (IL)‐8, a potent chemoattractant and activator of neutrophils, has been implicated to have a major role in the pathogenesis of gastric mucosal injury by Helicobacter pylori infection. We examined the relationship between cytotoxicity and IL‐8 secretion induced by H. pylori. Furthermore, whether the vacuolating cytotoxin of H. pylori mediates IL‐8 secretion from gastric epithelial cell lines was examined. Among the inflammatory cytokines, messages for IL‐6, IL‐8 and transforming growth factor‐β 1 were produced by gastric cancer (MKN45) cells in response to exposure to the cytotoxic strain of H. pylori. MKN45 incubated with the viable cytotoxic strain of H. pylori. secreted IL‐8. In contrast, the supernatant of neither the cytotoxic nor the non‐cytotoxic strain induced IL‐8 secretion. There was no correlation between IL‐8 secretion and the intensity of cytotoxicity. In conclusion, these findings suggest that IL‐8 secretion from MKN45 induced by H. pylori is mediated by factors other than cytotoxicity.