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Functional and phenotypical activation of leucocytes in inflamed human colonic mucosa
Author(s) -
OSHITANI NOBUHIDE,
SAWA YOSHINORI,
HARA JUNICHI,
ADACHI KENJI,
NAKAMURA SHIRO,
MATSUMOTO TAKAYUKI,
ARAKAWA TETSUO,
KUROKI TETSUO
Publication year - 1997
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1997.tb00376.x
Subject(s) - calprotectin , colitis , medicine , s100a9 , inflammation , eosinophil , integrin alpha m , myeloperoxidase , macrophage , intestinal mucosa , eosinophil cationic protein , immunology , pathology , flow cytometry , in vitro , chemistry , inflammatory bowel disease , biochemistry , disease , asthma
Infiltrating leucocytes are activated to generate reactive oxygen species or to produce several molecules in inflamed colonic mucosa. To clarify the phenotypical and functional properties of activating cells in colitic mucosa, 23 patients with ulcerative colitis and 13 controls were studied using a combined method for determining in situ nitroblue tetrazolium reducing activity and immunohisto‐chemical characterization. Antibodies 25F9 (anti‐macrophage), EG2 (anti‐eosinophil cationic protein), MAC387 (anti‐calprotectin, expressed by activated myeloid‐histiocytes lineage), and MAC‐1 (anti‐CD11b) were used. The proportion of EG2, calprotectin, and CD11b‐positive cells were significantly increased in inflamed mucosa. The proportion of EG2, calprotectin, and CD11b‐positive cells significantly correlated with the histological degree of inflammation. Proportion of EG2‐positive cells but not calprotectin nor CD11b‐positive cells was significantly correlated with nitroblue tetrazolium reducing activity. Aggregated cells reducing nitroblue tetrazolium seen in severely inflamed mucosa were found to be EG2 positive. Most of the calprotectin‐positive cells were 25F9 negative. In addition to activation of neutrophils and macrophages, eosinophil activation has been shown to be involved in inflamed colonic mucosa.