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A quantitative assessment of serum chylomicron by light scattering intensity: Application to the intestinal fat absorption test
Author(s) -
TAZUMA SUSUMU,
MIURA HIROYUKI,
HIRANO NAOMICHI,
HATTORI YOSHIHIRO,
KAJIHARA TSUYOSHI,
TSUCHIMOTO DENYA,
MIYAKE HIROAKI,
NISHIOKA TOMOJI,
HYOGO HIDEYUKI,
NAKAO SEIJI,
YAMASHITA GUNJI,
KAJIYAMA GORO
Publication year - 1997
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1997.tb00358.x
Subject(s) - chylomicron , triolein , malabsorption , triglyceride , absorption (acoustics) , medicine , endocrinology , intensity (physics) , gastroenterology , chromatography , chemistry , biochemistry , lipoprotein , cholesterol , very low density lipoprotein , materials science , physics , quantum mechanics , lipase , composite material , enzyme
A novel fat absorption test to clarify the malabsorption syndrome was developed using a micronephelometric technique and compared with the classic conventional technique using 131 I‐triolein. An integrity of time‐sequential light scattered from chylomicron‐related turbidity in serum was determined between 0 and 300 min after butter fat load, being expressed in terms of the light scattering intensity (LSI). A good correlation was obtained between LSI and the serum level of chylomicron‐triglyceride determined by an ultracentrifugation technique ( r =0.819, P < 0.001). The maximal LSI was consistently observed at 180 min after administration of a test meal in the normal group ( n = 39), whereas the malabsorption syndrome group ( n = 35) was distinctly different and could be further classified according to four patterns of LSI changes. In addition, an inverse correlation was found between this fat absorption test and the 131 I‐triolein absorption test. It was concluded that the micronephelometric technique which does not use a radionuclide is advantageous in its simple and safe evaluation of fat malabsorption syndrome.