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Studies on the hypomethylation of c‐myc, c‐Ha‐ras oncogenes and histopathological changes in human gastric carcinoma
Author(s) -
FANG JINGYUAN,
ZHU SHUNSHI,
XIAO SHUDONG,
JIANG SHAOJI,
SHI YAO,
CHEN XIAOYU,
ZHOU XIAOMEI,
QIAN LIANFANG
Publication year - 1996
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1996.tb00040.x
Subject(s) - medicine , gastric carcinoma , carcinoma , cancer research , pathology , oncology , cancer
In order to study the status of DNA methylation of specific oncogenes and the relationship between them and the pathological changes in gastric carcinoma, we analysed the methylated status of c‐myc, c‐Ha‐ras oncogenes by Southern blot hybridization. Genomic DNA from cancerous, paracancerous and non‐cancerous areas of surgically resected specimens were examined in 22 cases of advanced human gastric carcinoma. Specimens were digested by the restriction endonucleases MspI/HpaII, which are able to cleave between methylated and non‐methylated cytosine at their nucleotide recognition site the DNA 5′‐CCGG sequence, and were hybridized with c‐myc, c‐Ha‐ras oncogene probes. Moreover, the corresponding pathological changes in gastric carcinoma were observed. The results showed that c‐myc, c‐Ha‐ras oncogenes from cancerous (10/22, 5/10) and paracancerous areas (13/22, 4/10) were hypomethylated and that there was no significant relationship between them and the histopathological changes.