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Effect of nicotine in migration and proliferation of rabbit gastric mucosal cells in a culture cell model
Author(s) -
SATO N.,
WATANABE S.,
HIROSE M.,
WANG XE.,
MAEHIRO K.,
MURAI T.,
KOBAYASHI O.,
NAGAHARA A.,
OGIHARA T.,
KITAMI N.
Publication year - 1994
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1994.tb01305.x
Subject(s) - nicotine , medicine , bromodeoxyuridine , vacuole , wound healing , gastric mucosa , cell culture , cell , parietal cell , pathology , pharmacology , andrology , surgery , stomach , microbiology and biotechnology , immunohistochemistry , biology , biochemistry , genetics , cytoplasm
The aim of this study was to assess the effects of nicotine on the gastric epithelial restoration using primary cultured rabbit gastric mucosal cell model. Confluent monolayer mucosal cell sheets consisting of mainly mucous cells were wounded using a rotating silicon tip. The process of restoration was monitored, and the size of wound was measured and analysed quantitatively. Artificial wounds recovered in 36 h in controls. The nicotine treatment (10 −5 , 10 −4 and 10 −3 mol/L) did not cause any effects on the process of wound repair. Bromodeoxyuridine (BrdU) positive cells appeared around the wound 24‐36 h after injury and then disappeared after the complete repair in controls and also in nicotine‐treated groups. However, in the morphological observation, numerous vacuoles were detected in parietal cells of nicotine‐treated groups. This effect of nicotine was reversible by removing nicotine from the medium. Present data suggest that nicotine has no direct effects on the mucosal restoration but might have an effect on the structure and function of parietal cells.