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Increased sister chromatid exchange frequency in peripheral lymphocytes of hepatocellular carcinoma patients
Author(s) -
WANG LY.,
HUANG SJ.,
LIAW YF.,
CHEN CJ.
Publication year - 1993
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1993.tb01670.x
Subject(s) - medicine , hepatocellular carcinoma , sister chromatid exchange , lymphocyte , hepatitis b virus , carcinogen , hepatitis b , sister chromatids , immunology , gastroenterology , pathology , virus , biology , biochemistry , in vitro , chromosome , gene
Hepatocellular carcinoma (HCC) has been documented to be associated with a multifactorial aetiology including hepatitis B virus (HBV), hepatitis C virus, exposure to aflatoxin, tobacco and alcohol. With the specific aim to examine the relationship between HCC and frequency of sister chromatid exchange (SCE) in peripheral lymphocytes as an indicator of integral carcinogen exposure, a total of 14 newly diagnosed untreated HCC patients and 13 healthy controls were studied. Study subjects were matched on age, sex, cigarette smoking and alcohol intake. Peripheral blood samples were collected using heparinized vacuum syringes. Lymphocytes were cultured in RPMI 1640 medium containing fetal calf serum and phytohaemagglutin. After lymphocytes were cultured for 24 h, 5‐bromo‐2‐deoxy uridine was added to the medium and colcemid was added after 70 h. Cells were harvested and fixed in methanol‐acetic acid (3 : 1) solution after 72 h. Cells were first stained with 33258 Hoechst and restained with Giemsa. Photographs were taken from at least 32 lymphocytes at the second metaphase of each study subject. The frequency of SCE was scored by two readers blindly. There was a significantly increased SCE frequency in peripheral lymphocytes of HCC patients than healthy controls, with a mean ± standard deviation of 15.1 ± 4.4 and 8.9 ± 2.7 SCE per metaphase II, respectively. The increased SCE frequency in lymphocytes of HCC patients may be due to the chronic HBV infection, exposure to chemical hepatocarcinogens or both.