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Genotypic subtyping of hepatitis C virus
Author(s) -
CHAYAMA KAZUAKI,
TSUBOTA AKIHITO,
ARASE YASUJI,
SAITOH SATOSHI,
KOIDA ISAO,
IKEDA KENJI,
MATSUMOTO TOYOMI,
KOBAYASHI MARIKO,
IWASAKI SATOMI,
KOYAMA SHIMA,
MORINAGA TSUTO,
KUMADA HIROMITSU
Publication year - 1993
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1993.tb01507.x
Subject(s) - subtyping , virology , genotype , hepatitis c virus , typing , polymerase chain reaction , medicine , primer (cosmetics) , nucleic acid sequence , nucleotide , virus , biology , genetics , gene , chemistry , organic chemistry , computer science , programming language
Four subtypes of hepatitis C virus (HCV), Pt(I), K1(II), K2a(III) and K2b(IV), have been suggested based on the nucleotide sequences of the non‐structural (NS) 5 region. A fifth subtype from Japanese patients, Tr(V), which shows a less than 68% homology in nucleotide sequence when compared with other subtypes has been identified. A one‐step method which enables a quick determination of subtype using polymerase chain reaction with a mixed primer set deduced from the sequence of each subtype has been developed. Using this technique, the subtypes of 418 out of 478 Japanese patients (87.4%) were determined. The incidence of each subtype in Japan was as follows: K1(II), 307 (73.4%); K2a(III), 74 (17.7%); K2b(IV), 28 (6.7%); and Tr(V), 3 (0.7%). This one‐step subtyping technique should be useful for studying the epidemiology or biology of the HCV.