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Enhanced production of leukotriene B 4 by peripheral blood mononuclear cells in patients with fulminant hepatitis
Author(s) -
ASANO F.,
MORIWAKI H.,
SHIRATORI Y.,
SHIMAZAKI M.,
SAKAI T.,
KOSHINO Y.,
MURAKAMI N.,
SUGIHARA J.,
OHNISHI H.,
SAITO K.,
MUTO Y.
Publication year - 1993
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1993.tb01191.x
Subject(s) - peripheral blood mononuclear cell , medicine , leukotriene b4 , fulminant hepatitis , leukotriene , immunology , radioimmunoassay , fulminant , stimulation , hepatitis , inflammation , biology , in vitro , biochemistry , asthma
The production rate of leukotriene B 4 (LTB4) was measured using peripheral blood mononuclear cells (PBMC) in patients with fulminant hepatitis (FH) or other liver diseases. LTB4 in the culture media of PBMC under stimulation with Ca‐ionophore was fractionated by HPLC and measured by radioimmunoassay. The production rate of LTB4 was elevated in 16 of 17 FH patients (3.3 ± 0.2 ng/10 6 cells for 5 min), while the production was below detectable level in patients with acute or chronic hepatitis and in healthy controls. In FH patients, the highest production rate of LTB4 was observed in the initial period of the disease. Enhanced LTB4 production may indicate the primed state of PBMC — the primed mononuclear cells are regarded as participating in the development of massive liver necrosis and of other organ failures in FH.