Premium
Effects of arachidonic acid metabolites and interleukin‐1 on platelet activating factor production by hepatic sinusoidal endothelial cells from mice
Author(s) -
MIZOGUCHI YASUHIRO,
ICHIKAWA YUZO,
KIOKA KIYOHIDE,
KAWADA NORIFUMI,
KOBAYASHI KENZO,
YAMAMOTO SUKEO
Publication year - 1991
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1991.tb01479.x
Subject(s) - arachidonic acid , medicine , endocrinology , platelet activating factor , leukotriene , leukotriene b4 , platelet , eicosanoid , incubation , prostaglandin e2 , immune system , leukotriene c4 , biology , inflammation , immunology , biochemistry , enzyme , asthma
The effects of interleukin‐1 (IL‐1) and arachidonic acid metabolites, prostaglandin (PG) E 1 , leukotriene (LT) B 4 and LTC 4 , on the amount of platelet activating factor (PAF) produced and released by mouse hepatic sinusoidal endothelial cells were investigated. When hepatic sinusoidal endothelial cells were incubated with 1 μg/mL of calcium ionophore (CaI) A23187, PAF production increased until 20 min after the start of incubation, but when the cells were incubated with PGE 1 and CaI A23187, PAF production was significantly suppressed. On the other hand, when hepatic sinusoidal endothelial cells were incubated with IL‐1β, LTB 4 or LTC 4 alone, PAF production significantly increased compared with that of the cells incubated without these substances. However, when the cells were incubated with PGE 1 alone or with IL‐1β, LTB 4 or LTC 4 and CaI A23187, these effects were not observed. These results indicated that PAF produced by hepatic sinusoidal endothelial cells is affected by IL‐1 and arachidonic acid metabolites, suggesting that immune and inflammatory reactions in these cells may be regulated by chemical mediators produced by the cells themselves.