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Molecular epidemiology of hepatitis B virus in Indonesia
Author(s) -
SASTROSOEWIGNJO RETNO I.,
SANDJAJA BERNARDUS,
OKAMOTO HIROAKI
Publication year - 1991
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1991.tb00894.x
Subject(s) - genotype , virology , hepatitis b virus , biology , molecular epidemiology , nucleic acid sequence , polymerase chain reaction , gene , genetics , homology (biology) , hepadnaviridae , virus
The S ‐gene sequences of hepatitis B virus (HBV) from 22 carriers in several islands of Indonesia were amplified by polymerase chain reaction, and X ba I‐S pe l fragments corresponding to nucleotides 93–529 (437 base pairs) in the S gene were sequenced. The 22 sequences, along with the 5 reported sequences from Indonesia, were compared with each other, and with the corresponding sequences of 20 clones from other countries including China, France, Great Britain, Japan, Kenya, Papua New Guinea, Philippines, USA and USSR. When the 27 HBV DNA clones of various subtypes from Indonesia were classified by the homology in the nucleotide sequence into the five genotypes, twelve belonged to genotype B (subtype adw 7 and ayw 5), 13 to genotype C ( adw 1, adr 10, ayr 1 and ar 1), and 2 to genotype D ( ayw ); none belonged to genotype A or E. Different subtypes of clones in the same genotype indicated that point mutations inducing d ‐to‐ y or w ‐to‐ r phenotypic changes would be common among Indonesian carriers. Comparison of the translation products of X ba l‐S pe l fragments, now available for 47 HBV DNA clones of different genotypes (A 4; B 14; C 21; D 7; E 1), identified several amino acids characteristic to or influenced by the five genotypes as well as those highly conserved by clones of different genotypes.