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Collagen synthesis by cultured rat liver cells isolated from chronically alcohol‐treated rats
Author(s) -
TAKASE SHUJIRO,
ENYAMA KEIKO,
TAKADA AKIRA
Publication year - 1990
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1990.tb01419.x
Subject(s) - hydroxyproline , proline , endocrinology , medicine , intracellular , type i collagen , alcohol , hepatic stellate cell , pathogenesis , cell culture , hepatocyte , biochemistry , in vitro , chemistry , biology , amino acid , genetics
Incorporation rates of 14 C‐proline into collagen hydroxyproline in cultured Ito cells and hepatocytes isolated from chronically alcohol‐treated rats were studied in order to clarify the role of Ito cells in the development of alcoholic liver fibrosis pathogenesis. In the cultured Ito cells isolated from alcohol‐treated rats, prolyl hydroxylase activity significantly increased. Total collagen synthesis tended to increase in the alcohol group, and the increase in intracellular intact collagen was statistically significant. More than half of the 14 C‐radioactivity in intact collagen in cultured Ito cells from control rats was found in collagen other than types I and III collagen (mainly type IV collagen). In Ito cells from alcohol‐treated rats, synthesis of collagen other than type I and III significantly increased and type I collagen synthesis tended to be decreased. No significant difference was found in collagen synthesis between the cultured hepatocytes from alcoholic and control rats. These results suggest that chronic alcohol consumption stimulates collagen synthesis in Ito cells, especially type IV collagen. This stimulation of Ito cells may play a role in the development of alcoholic liver fibrosis.