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Biological potency of neurotensin metabolites in vivo: Importance of alcohol ‘fixation’ of blood
Author(s) -
SHULKES ARTHUR,
FLETCHER DAVID R.,
HARDY KENNETH J.
Publication year - 1989
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1989.tb01740.x
Subject(s) - neurotensin , in vivo , potency , medicine , alcohol , ethanol , peptide , biochemistry , ileum , endocrinology , in vitro , neuropeptide , biology , receptor , microbiology and biotechnology
Neurotensin (NT), a 13‐amino acid peptide, is released from the ileum following a meal. It is metabolized principally by the kidney and in the circulation to N ‐terminal fragments and apparently rapidly degraded C ‐terminal fragments. The present study was designed to compare the biological activity (plasma pancreatic polypeptide response) and the clearance kinetics of NT(1‐13), the N ‐terminal fragment NT(1‐11) and the C ‐terminal fragment NT(8‐13). To measure accurately the circulating concentrations of short‐lived NT fragments in the circulation, a method was devised of collecting blood directly into alcohol (‘alcohol fixation’).