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Metabolic activation of polycyclic aromatic hydrocarbons by human colonic mucosa and Bacteroides fragilis
Author(s) -
TASICH M.,
HAFNER L.,
PIPER D. W.,
STIEL D.
Publication year - 1986
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1986.tb01756.x
Subject(s) - bacteroides fragilis , pyrene , carcinogen , anthracene , microsome , s9 fraction , chemistry , ames test , biochemistry , microbiology and biotechnology , bacteria , enzyme , salmonella , biology , organic chemistry , genetics , antibiotics
The Ames Salmonella mutagenicity assay has been used to assess the metabolic activation of the following polycyclic aromatic hydrocarbons by human colonic microsomes (S9) and a cell‐free extract of Bacteroides fragilis (Bf): 2‐aminoanthracene, 1‐naphthylamine, 2‐naphthylamine, 2‐aminofluorene, 2‐acetylaminofluorene, anthracene, benzo(a)pyrene, 3‐methylcholanthrene, 7, 12‐dimethylbenzanthracene, acridine, 9‐aminoacridine and 3‐methylindole. 2‐Aminoanthracene and 2‐aminofluorene were the only compounds activated. In both cases, activation was dose‐dependent and 2‐aminofluorene exhibited synergistic activation by S9+Bf, as has previously been demonstrated with 2‐aminoanthracene. The organospecific aliphatic colonic carcinogen, 1, 2‐dimethylhydrazine was not activated in this system. Metabolic activation by S9+Bf is thus restricted to aromatic compounds with three rings and an amino group in position 2. These findings are consistent with enzymic substrate specificity, and are compatible with an enzymic basis for activation of polycyclic aromatic hydrocarbons by B. fragilis , present in large concentrations in the colonic lumen, and colonic microsomes.