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Liver microvascular flow in rats: Quantitation by laser Doppler flowmetric technique and effects of sodium dehydrocholate
Author(s) -
KOO ANTHONY
Publication year - 1986
Publication title -
journal of gastroenterology and hepatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.214
H-Index - 130
eISSN - 1440-1746
pISSN - 0815-9319
DOI - 10.1111/j.1440-1746.1986.tb00775.x
Subject(s) - medicine , cannula , in vivo , hyperaemia , sodium , blood flow , laser doppler velocimetry , common bile duct , surgery , chemistry , biology , microbiology and biotechnology , organic chemistry
An in vivo in situ liver preparation in anaesthetized male Sprague‐Dawley rats was devised for simultaneous measurements of hepatic microvascular flow and bile flow. The micro‐vascular flow on the surface of the liver was continuously recorded by laser Doppler flowmetric technique whilst bile flow was quantitated by connecting the bile duct cannula to a drop counter. Sodium dehydrocholate was infused (0.2 ml in 2–5 min) both via a cannula in the splenic artery and via a cannula in the superior mesenteric vein. Results showed that sodium dehydrocholate, given by either route, increased the liver microvascular flow and bile flow. A cross‐plot between the hyperaemic and hypercholeretic responses corresponding to the same dose of sodium dehydrocholate revealed a significant correlation ( P < 0.01) between the sodium dehydro‐cholate‐induced hypercholeresis and hyperaemia. Furthermore, the increase of microvascular flow occurred significantly ( P < 0.05) earlier than the hypercholeretic response. Results thus suggested that an increase of plasma blood flow was essential for the development of hypercholeresis induced by sodium dehydrocholate.