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Immunoadjuvant chaperone, GRP170, induces ‘danger signals’ upon interaction with dendritic cells
Author(s) -
Manjili Masoud H,
Park JunEui,
Facciponte John G,
Wang XiangYang,
Subjeck John R
Publication year - 2006
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/j.1440-1711.2006.01418.x
Subject(s) - immunoadjuvant , cd86 , microbiology and biotechnology , cd40 , secretion , dendritic cell , chemistry , immune system , downregulation and upregulation , chaperone (clinical) , mhc class ii , antigen , biology , immunology , major histocompatibility complex , t cell , in vitro , biochemistry , medicine , gene , cytotoxic t cell , pathology
When chaperoning tumour antigens, glucose‐regulated protein 170 (GRP170) is capable of inducing effective antitumour immune responses. In the present study, we determined whether such immunoadjuvant properties of GRP170 also involve the ability to induce ‘danger signals’ through interaction with APC. We prepared recombinant GRP170 in the baculovirus expression system with low endotoxin concentration at which LPS did not have any effect on dendritic cells (DC). We showed that GRP170 binds DC in a receptor‐mediated fashion and induces DC to upregulate the expression of MHC class II, CD86 and CD40 molecules, and to secrete pro‐inflammatory cytokines. GRP170 also induced expression of CD40 molecules in a B16F10 cell line, whereas LPS failed to do so. These findings show that GRP170 acts as a danger signal through its interaction with DC, regardless of its endotoxin component.