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Coinoculation with hepatitis B surface and core antigen promotes a Th1 immune response to a multiepitopic protein of HIV‐1
Author(s) -
Iglesias Enrique,
Thompson Rafael,
Carrazana Yamilka,
Lobaina Yadira,
García Daymir,
Sánchez Jorge,
García José,
Cruz Otto,
Brown Emma,
Martin Alejandro,
Muzio Verena L,
Aguilar Julio C
Publication year - 2006
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/j.1440-1711.2005.01408.x
Subject(s) - recombinant dna , immune system , adjuvant , epitope , antigen , virology , ctl* , biology , immunization , immunology , heterologous , cd8 , biochemistry , gene
It has been defined that strong and multispecific cellular immune responses correlate with a better prognosis during the course of chronic diseases. A cross‐enhancing effect on the resulting immune response obtained by the coadministration of recombinant hepatitis B virus (HBV) surface and core Ag was recently observed. With the objective of studying the effect of such Ag on the immune response to coinoculated heterologous Ag and vice versa, several formulations containing the recombinant HBV Ag and a multiepitopic protein (CR3) composed by CTL and Th epitopes from HIV‐1 were evaluated by s.c. and mucosal administration. Combinations of two and three Ag were evaluated for cellular and humoral immune responses. The results showed that the best Ag combination for nasal immunization was the mixture comprising the CR3 recombinant HIV protein and both HBV Ag. Similarly, it was also the best formulation for s.c. immunization in aluminium phosphate adjuvant. In conclusion, it is possible to induce a Th1 stimulation of the cellular immune response specific for a HIV‐based recombinant protein by formulating this Ag with the recombinant HBV Ag.