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RGDS peptide inhibits activation of lymphocytes and adhesion of activated lymphocytes to human umbilical vein endothelial cells in vitro
Author(s) -
Pozzetto Ubaldo,
Aguzzi Maria Simona,
Maggiano Nicola,
Scala Enrico,
Capelli Arnaldo,
Castagneto Marco,
Capogrossi Maurizio C,
Citterio Franco,
Serino Francesco,
Facchiano Antonio
Publication year - 2005
Publication title -
immunology and cell biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.999
H-Index - 104
eISSN - 1440-1711
pISSN - 0818-9641
DOI - 10.1111/j.1440-1711.2005.01300.x
Subject(s) - umbilical vein , microbiology and biotechnology , cell adhesion , peripheral blood mononuclear cell , extracellular matrix , chemistry , integrin , lymphocyte , adhesion , immunology , endothelial stem cell , in vitro , cell , biology , biochemistry , organic chemistry
The Arg‐Gly‐Asp (RGD) motif is known to mediate cell adhesion to several extracellular matrix components as well as cell–cell interactions. In the present study, we investigated whether the RGDS peptide interferes with cell–cell recognition‐based events such as allogeneic activation of PBMC and PBMC adhesion to human umbilical vein endothelial cells (HUVEC). We show here for the first time, to our knowledge, that RGDS significantly inhibits adhesion of activated PBMC to HUVEC; in addition, RGDS inhibits PBMC allogenenic activation in human mixed lymphocyte reaction assays. Caspases played a pivotal role in both events, because preventing their activation abolished or strongly reduced the observed inhibitory effect. The RGDS antirecognition effect was strongly increased by pretreatment of HUVEC with RGDS, which affected mostly T lymphocyte adhesion to HUVEC. These results indicate that PBMC allogeneic activation, as well as reciprocal recognition between activated PBMC and endothelial cells, are RGDS‐dependent events that occur through a dual effect involving anti‐adhesive and caspase‐dependent mechanisms. These data suggest a potential role of RGDS in cell‐mediated immunity, inflammation and organ transplantation.