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Muscle development and differentiation in the urodele A mbystoma mexicanum
Author(s) -
Banfi Serena,
Monti Laura,
Acquati Francesco,
Tettamanti Gianluca,
Eguileor Magda,
Grimaldi Annalisa
Publication year - 2012
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2012.01338.x
Subject(s) - myogenesis , myf5 , notochord , ambystoma mexicanum , biology , myocyte , anatomy , amphibian , xenopus , skeletal muscle , microbiology and biotechnology , population , vertebrate , zebrafish , myod , embryo , axolotl , embryogenesis , regeneration (biology) , genetics , gene , ecology , demography , sociology
Muscle differentiation has been widely described in zebrafish and X enopus, but nothing is known about this process in amphibian urodeles. Both anatomical features and locomotor activity in urodeles are known to show intermediate features between fish and anurans. Therefore, a better understanding of myogenesis in urodeles could be useful to clarify the evolutionary changes that led to the formation of skeletal muscle in the trunk of land vertebrates. We report here a detailed morphological and molecular investigation on several embryonic stages of A mbystoma mexicanum and show that the first differentiating muscle fibers are the slow ones, originating from a myoblast population initially localized close to the notochord that forms a superficial layer on the somitic surface afterwards. Subsequently, fast fibers differentiation ensues. We also identified and cloned A . mexicanum M yf5 as a muscle‐specific transcriptional factor likely involved in urodele muscle differentiation.