Premium
Identification of a yolk sac cell population with hematopoietic activity in view of CD45/c‐Kit expression
Author(s) -
Yamasaki Shoutarou,
Nobuhisa Ikuo,
Ramadan Ahmed,
Taga Tetsuya
Publication year - 2011
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2011.01293.x
Subject(s) - yolk sac , identification (biology) , haematopoiesis , biology , population , expression (computer science) , microbiology and biotechnology , andrology , genetics , medicine , embryo , stem cell , botany , computer science , programming language , environmental health
During murine embryonic development, primitive hematopoiesis occurs in the yolk sac (YS). Recent studies have shown that the YS also harbors definitive hematopoietic activity. However, the population of YS cells contributing to definitive hematopoiesis has not been identified. In this study, we characterized the hematopoietic cell populations in the YS of mouse embryos from E9.5 to E14.5 in view of the expression profiles of CD45 and c‐Kit. The YS cells from E9.5 to E11.5 could be divided into six populations: CD45 − c‐Kit − , CD45 − c‐Kit low , CD45 − c‐Kit high , CD45 low c‐Kit high , CD45 high c‐Kit high and CD45 high c‐Kit very low . Among these populations, CD45 low c‐Kit high cells showed the highest multilineage hematopoietic colony‐forming activity. Later in development, the YS cells from E12.5 to E14.5 lost the second and fourth populations (i.e., they retained CD45 − c‐Kit − , CD45 − c‐Kit high , CD45 high c‐Kit high and CD45 high c‐Kit very low cells), and concurrently with the disappearance of the CD45 low c‐Kit high population, no significant hematopoietic activity was found in any of the populations on and after E12.5. CD45 low c‐Kit high YS cells, which had a round morphology with a large nucleus, possessed the ability to differentiate into myeloid and B lymphoid cells when cultured with stromal cells. These findings suggest that CD45 low c‐Kit high YS cells include more undifferentiated cells than the other YS cell populations and possess in vitro potency to differentiate into multilineage hematopoietic cells. Furthermore, this cell population disappears from the YS at around E12.5, when the site of hematopoiesis has already shifted to the fetal liver and the placenta.