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Regional expression of ADAM19 during chicken embryonic development
Author(s) -
Yan Xin,
Lin Juntang,
Markus Annett,
Rolfs Arndt,
Luo Jiankai
Publication year - 2011
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2010.01238.x
Subject(s) - dorsal aorta , biology , embryo , metalloproteinase , embryogenesis , embryonic stem cell , immunohistochemistry , western blot , neurite , morphogenesis , reverse transcription polymerase chain reaction , microbiology and biotechnology , anatomy , pathology , matrix metalloproteinase , messenger rna , immunology , gene , genetics , medicine , in vitro
ADAM19 (also named meltrin β) is a member of the ADAM (a disintegrin and metalloprotease) family of metalloproteases and is involved in morphogenesis and tissue formation during embryonic development. In the present study, chicken ADAM19 is cloned by reverse transcription–polymerase chain reaction and identified by sequencing. Its expression patterns in different parts of the developing chicken embryo are investigated by Western blot analysis and immunohistochemistry. Results show that ADAM19 protein is widely expressed in chicken embryos. It is detectable in the central nervous system, including the brain, spinal cord, cochlea, and retina. Furthermore, ADAM19 protein is also found in other tissues and organs such as digestive organs, the thymus, the lung bud, the dorsal aorta, the kidney, the gonad, muscles, and in the feather buds. All these data suggest that ADAM19 plays an important role in the embryonic development of chicken.