Premium
Development of Ca 2+ signaling mechanisms and cell motility in presumptive ectodermal cells during amphibian gastrulation
Author(s) -
Takano Kazuhiro,
Obata Shuichi,
Komazaki Shinji,
Masumoto Mika,
Oinuma Tsutomu,
Ito Yuzuru,
Ariizumi Takashi,
Nakamura Hiroaki,
Asashima Makoto
Publication year - 2011
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2010.01220.x
Subject(s) - amphibian , gastrulation , motility , microbiology and biotechnology , biology , ectoderm , chemistry , anatomy , zoology , embryo , embryogenesis , ecology
This study investigated the development of Ca 2+ signaling mechanisms and their role in initiating morphogenetic cell movement in the presumptive ectoderm of Japanese newt ( Cynops pyrrhogaster ) during gastrulation. Histochemical staining using fluorescently labeled ryanodine and dihydropyridine probes revealed that dihydropyridine receptor (L‐type Ca 2+ channels) appeared in stage 12b embryos, while ryanodine receptors were expressed in both stage 11 and 12b embryos. Transmission electron microscopy of stage 12b embryos showed abundant peripheral couplings, which are couplings of the endoplasmic reticulum and cell membrane with an approximate 12 nm gap. Caffeine increased the intracellular free Ca 2+ concentration ([Ca 2+ ] i ) in presumptive ectodermal cells isolated from both stage 11 and 12b embryos, while (±)‐Bay K 8644 ((±)‐BayK) increased [Ca 2+ ] i in cells isolated from stage 12b embryos, but not in cells isolated from stage 11 embryos. Dantrolene and nifedipine completely inhibited increases in [Ca 2+ ] i after treatment with caffeine and (±)‐BayK, respectively. Caffeine activated the motility of cells isolated from both stage 11 and 12b embryos, but (±)‐BayK only activated the motility of cells isolated from stage 12b embryos. These findings suggested that formation of the Ca 2+ ‐induced Ca 2+ release system in presumptive ectodermal cells during gastrulation plays an important role in the initiation and execution of epibolic extension.