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Molecular characterization and expression analysis of ADAM12 during chicken embryonic development
Author(s) -
Lin Juntang,
Luo Jiankai,
Redies Christoph
Publication year - 2010
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2010.01212.x
Subject(s) - gene isoform , biology , complementary dna , in situ hybridization , embryogenesis , mesoderm , reverse transcription polymerase chain reaction , disintegrin , neural crest , microbiology and biotechnology , embryonic stem cell , messenger rna , metalloproteinase , genetics , embryo , gene , matrix metalloproteinase
ADAM12 is a member of the disintegrin and metalloprotease (ADAM) family of molecules, which consist of multiple domains. ADAM12 is involved in different physiological and pathological processes. In the present study, full‐length sequences of two chicken ADAM12 isoforms were cloned and identified by reverse transcription–polymerase chain reaction (RT–PCR), rapid amplification of cDNA ends methods and bioinformatics analysis. The long isoform consists of all domains characteristic for ADAMs and is strongly expressed in different tissues, whereas the short isoform lacks large parts of the metalloprotease and disintegrin domains and is only expressed weakly. Results from semi‐quantitative RT–PCR show that the complete ADAM12 is stably expressed throughout chicken embryonic development, while the short isoform is only regionally detectable in the lung and brain. Results from in situ hybridization show that chicken ADAM12 is expressed exclusively in tissues and organs derived from the neural tube, the neural crest or the mesoderm, with a highly regulated spatiotemporal expression pattern. Our data confirm and extend studies of ADAM12 in other species, and suggest that ADAM12 may play a role in the development of several organs, including the formation of feather buds.