Premium
Gene manipulation of chick embryos in vitro , early chick culture, and long survival in transplanted eggs
Author(s) -
Tanaka Jun,
Harada Hidekiyo,
Ito Kodai,
Ogura Toshihiko,
Nakamura Harukazu
Publication year - 2010
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2010.01198.x
Subject(s) - electroporation , embryo , biology , transgene , transfection , in vitro , microbiology and biotechnology , gene , transposable element , gene expression , genetics , andrology , mutant , medicine
We introduce a revolutionary gene transfer system in chick: transfect chick embryos at early developmental stage by electroporation in vitro , Early Chick (EC) culture, and transplant to the egg to let the embryo survive until E5.5. Referring to the fate map, we could target the tissues of transfection, or transfect large areas of the embryo. We could get tissue‐specific expression of a transgene by tissue‐specific promoter. This method is very convenient and rapid, but allows us to get stable expression of the transgene in combination with transposon system.