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Infrared laser‐mediated local gene induction in medaka, zebrafish and Arabidopsis thaliana
Author(s) -
Deguchi Tomonori,
Itoh Mariko,
Urawa Hiroko,
Matsumoto Tomohiro,
Nakayama Sohei,
Kawasaki Takashi,
Kitano Takeshi,
Oda Shoji,
Mitani Hiroshi,
Takahashi Taku,
Todo Takeshi,
Sato Junichi,
Okada Kiyotaka,
Hatta Kohei,
Yuba Shunsuke,
Kamei Yasuhiro
Publication year - 2009
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2009.01135.x
Subject(s) - oryzias , biology , zebrafish , danio , arabidopsis thaliana , gene expression , gene , reporter gene , caenorhabditis elegans , arabidopsis , microbiology and biotechnology , regulation of gene expression , genetics , mutant
Heat shock promoters are powerful tools for the precise control of exogenous gene induction in living organisms. In addition to the temporal control of gene expression, the analysis of gene function can also require spatial restriction. Recently, we reported a new method for in vivo , single‐cell gene induction using an infrared laser‐evoked gene operator (IR‐LEGO) system in living nematodes ( Caenorhabditis elegans ). It was demonstrated that infrared (IR) irradiation could induce gene expression in single cells without incurring cellular damage. Here, we report the application of IR‐LEGO to the small fish, medaka (Japanese killifish; Oryzias latipes ) and zebrafish ( Danio rerio ), and a higher plant ( Arabidopsis thaliana ). Using easily observable reporter genes, we successfully induced gene expression in various tissues in these living organisms. IR‐LEGO has the potential to be a useful tool in extensive research fields for cell/tissue marking or targeted gene expression in local tissues of small fish and plants.