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Interaction of ZFPIP with PBX1 is crucial for proper expression of neural genetic markers during Xenopus development
Author(s) -
Laurent Audrey,
Masse Julie,
Deschamps Stéphane,
Burel Agnes,
Omilli Francis,
RichardParpaillon Laurent,
Pellerin Isabelle
Publication year - 2009
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2009.01129.x
Subject(s) - xenopus , biology , neurogenesis , ectopic expression , neural development , microbiology and biotechnology , gene , gene expression , genetics
ZFPIP / Zfp462 has been recently identified as a new vertebrate zinc finger encoding gene whose product interacts with Pbx1. Previous work indicates that ZFPIP is maternally expressed in Xenopus laevis oocytes and plays a key role during the cleavage phase of embryogenesis. This early expression is followed by a zygotic expression which overlaps with the neural Pbx1 expression pattern, suggesting an interaction between these two partners during Xenopus neurogenesis. In order to test the physiological interaction between ZFPIP and Pbx1, we carried out a dominant negative assay in which the Pbx1 interacting domain of ZFPIP (ZFPIPp) was overexpressed in Xenopus laevis embryos. We observed that ZFPIPp ectopic expression led to abnormal en2 and N‐cam expression patterns, whereas krox‐20 expression was not affected. Furthermore, we showed that while ZFPIPp alone was localized in the nucleus of Cos‐7 cells, additional expression of Pbx1 induced a location of ZFPIPp at the perinuclear region of the cells. These overall data suggest that ZFPIP and Pbx1 could be partners and cooperate in the regulation of essential neural genes during Xenopus development.