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Spatio‐temporal expression of activating transcription factor 5 in the skeletal development of mouse limb
Author(s) -
Satake Hiroshi,
Ito Kazuo,
Takahara Masatoshi,
Furukawa Takashi,
Takagi Michiaki,
Ogino Toshihiko,
Shinomura Tamayuki
Publication year - 2009
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2009.01126.x
Subject(s) - sox9 , cartilage , activating transcription factor , in situ hybridization , transcription factor , biology , anatomy , microbiology and biotechnology , gene expression , gene , genetics
There are no data about the expression pattern of activating transcription factor 5 (Atf5) during limb development. The aim of the present study was to describe the expression of Atf5 throughout mouse limb development. Some specimens were examined for the expression of type II collagen α1 (Col2a1) , sex‐determining region Y‐related high‐mobility‐group‐box 9 ( Sox9) , and activating transcription factor 2 (Atf2) by in situ hybridization. ATDC5 cells, mouse limb buds, and epiphyseal cartilage were examined for the expression of Atf5α and Atf5β by quantitative real‐time PCR. Atf5 transcripts were detected in condensed mesenchymal cells, cartilage primordia, proliferative, prehypertrophic, and articular chondrocytes, but not in hypertrophic chondrocytes. Atf5 was predominantly expressed by osteoblasts in the primary spongiosa. At the postnatal stage, Atf5 was expressed in epiphyseal chondrocytes and osteoblasts lining the bone trabeculae. Spatial distribution of Col2a1 , Sox9 , and Atf2 transcripts closely corresponded to that of Atf5 within developing limbs, except for bony tissues. In cartilage, Atf5α is the dominant form in the embryonic stage as well as in the postnatal stage.