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Congenic method in the chick limb buds by electroporation
Author(s) -
Suzuki Takayuki,
Ogura Toshihiko
Publication year - 2008
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2008.01054.x
Subject(s) - electroporation , biology , embryonic stem cell , limb development , anatomy , microbiology and biotechnology , neuroscience , computational biology , gene , genetics , embryo
Electroporation is a powerful tool with which to study limb development. Limb development, however, remains an intricate series of events, requiring the precise dissection of developmental processes using relevant transgenes. In this review, we describe the anatomy of the limb field as the basis of targeted electroporation, and specific expression vectors are discussed. We share a useful protocol for electroporation of chick limb buds, and the expression pattern of enhanced green fluorescent protein in the limb buds is used to demonstrate relevant embryonic patterning. Finally, useful trouble‐shooting techniques are described.