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Method for electroporation for the early chick embryo
Author(s) -
Hatakeyama Jun,
Shimamura Kenji
Publication year - 2008
Publication title -
development, growth and differentiation
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.864
H-Index - 66
eISSN - 1440-169X
pISSN - 0012-1592
DOI - 10.1111/j.1440-169x.2008.01040.x
Subject(s) - electroporation , embryo , microbiology and biotechnology , biology , embryo culture , gastrulation , embryogenesis , in vitro , andrology , anatomy , genetics , gene , medicine
In vitro whole‐embryo culture of chick embryos, originally invented by New, has been widely used for studies of early embryogenesis. Here, a method for electroporation using the New culture and its derivatives is described, to achieve misexpression of exogenous gene in a temporally and spatially controlled manner in gastrulating chick embryos. Detailed information for the devices and procedures, and some experimental examples are presented.